The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Feb. 24, 2015

Filed:

Jul. 04, 2011
Applicants:

Diethard Mattanovich, Vienna, AT;

Martin Dragosits, Vienna, AT;

Brigitte Gasser, Vienna, AT;

Michael Maurer, Vienna, AT;

Michael Sauer, Vienna, AT;

Inventors:

Diethard Mattanovich, Vienna, AT;

Martin Dragosits, Vienna, AT;

Brigitte Gasser, Vienna, AT;

Michael Maurer, Vienna, AT;

Michael Sauer, Vienna, AT;

Assignee:
Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12P 21/04 (2006.01); C12P 1/02 (2006.01); C12N 15/00 (2006.01); C12N 15/74 (2006.01); C12N 15/81 (2006.01); C12N 15/67 (2006.01); C12N 15/80 (2006.01); C12P 21/02 (2006.01);
U.S. Cl.
CPC ...
C12N 15/815 (2013.01); C12N 15/67 (2013.01); C12N 15/80 (2013.01); C12P 21/02 (2013.01);
Abstract

The invention refers to a method of producing a recombinant polypeptide of interest (POI) in a cell culture, comprising genetically engineering a eukaryotic cell line—to specifically cause prolongation of the G2+M cell cycle phase in a pre-culture phase, and—to produce the POI in a producing phase following the pre-culture phase, a high producer cell line and cell culture as well as a method of increasing the yield of a recombinant POI production in a cell culture.


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