The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Aug. 12, 2014
Filed:
Apr. 03, 2008
Partha Hazra, Karnataka, IN;
Nitesh Dave, Karnataka, IN;
Vivekanandan Kannan, Karnataka, IN;
Sanjay Tiwari, Karnataka, IN;
Anuj Goel, Karnataka, IN;
Harish Iyer, Karnataka, IN;
Nita Roy, Karnataka, IN;
Krishnamurthy Venkatesan, Tamil Nadu, IN;
Anoop Vasudevan, Kerala, IN;
Anupama Jagadish, Karnataka, IN;
Goldy Sachdev, Karnataka, IN;
Mukesh Babuappa Patale, Maharashtra, IN;
Partha Hazra, Karnataka, IN;
Nitesh Dave, Karnataka, IN;
Vivekanandan Kannan, Karnataka, IN;
Sanjay Tiwari, Karnataka, IN;
Anuj Goel, Karnataka, IN;
Harish Iyer, Karnataka, IN;
Nita Roy, Karnataka, IN;
Krishnamurthy Venkatesan, Tamil Nadu, IN;
Anoop Vasudevan, Kerala, IN;
Anupama Jagadish, Karnataka, IN;
Goldy Sachdev, Karnataka, IN;
Mukesh Babuappa Patale, Maharashtra, IN;
Biocon Limited, Bangalore, IN;
Abstract
The invention relates to methods of separation and/or purification of impurities yielding a purified heterologous protein product devoid of related impurities or with substantially minimal quantities of such glycosylated impurities. More specifically, the invention relates to the identification of glycosylated forms of insulin analogues such as glargine impurities characterized post expression in yeast based systems such as. The invention also relates to methods used to clone gene encoding the protein insulin glargine; inserting the related gene in a suitable yeast host; producing culture of the recombinant strain, stimulating expression of the heterologous polypeptide, its secretion and purification post fermentation and related enzymatic conversions.