The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jul. 15, 2014
Filed:
Mar. 29, 2010
Nagaraj Govindappa, Karnataka, IN;
Komal Kanojia, Karnataka, IN;
Krishnamurthy Venkatesan, Tamil Nadu, IN;
Nitesh Dave, Karnataka, IN;
Mukesh Babuappa Patale, Maharashtra, IN;
Sanjay Tiwari, Karnataka, IN;
Kedarnath N. Sastry, Karnataka, IN;
Harish Iyer, Karnataka, IN;
Nagaraj Govindappa, Karnataka, IN;
Komal Kanojia, Karnataka, IN;
Krishnamurthy Venkatesan, Tamil Nadu, IN;
Nitesh Dave, Karnataka, IN;
Mukesh Babuappa Patale, Maharashtra, IN;
Sanjay Tiwari, Karnataka, IN;
Kedarnath N. Sastry, Karnataka, IN;
Harish Iyer, Karnataka, IN;
Biocon Limited, Bangalore, Karnataka, IN;
Abstract
The disclosure relates to method of reducing O-glycosylation levels of the insulin or insulin analog precursor molecule produced bysp. The present disclosure provides genetically engineered knock-out strains of methylotrophic yeast includingand especiallyby disruption of Protein mannosyl transferase (PMT) genes and rendering them capable of producing heterologous proteins with reduced glycosylation. Vectors, which comprise coding sequences for PMT1, PMT2, PMT4, PMT5, and PMT6 genes, for transforming methylotrophic yeasts are contemplated by the present disclosure. PMT inactivated strains of this disclosure have been deposited at MTCC, Chandigarh. The strains are PMT1/GS115 (MTCC 5515), PMT4/GS115 (MTCC 5516), PMT5/GS115 (MTCC 5517) and PMT6/GS115 (MTCC 5518).