The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Mar. 25, 2014

Filed:

Dec. 31, 2008
Applicants:

Xuefeng Yu, Yichang, CN;

Zhihong LI, Yichang, CN;

Minghua Yu, Yichang, CN;

Juan Yao, Yichang, CN;

Yan Zhang, Yichang, CN;

Inventors:

Xuefeng Yu, Yichang, CN;

Zhihong Li, Yichang, CN;

Minghua Yu, Yichang, CN;

Juan Yao, Yichang, CN;

Yan Zhang, Yichang, CN;

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12P 19/04 (2006.01); C12P 19/00 (2006.01); C12P 1/04 (2006.01); C12P 1/02 (2006.01); C12N 1/06 (2006.01); A01N 43/04 (2006.01);
U.S. Cl.
CPC ...
Abstract

The present invention provides a method for extracting glucan and mannan from the cell wall of a microorganism. Specifically, the method of the present invention in one embodiment comprises the steps of: a) treating the cells of the microorganism with an alkaline protease and an mannanase; b) separating the mixture from step a) into a heavy phase and a light phase; c) drying the heavy phase obtained from step b), obtaining the glucan preparation; and d) drying the light phase obtained from step b), obtaining the mannan preparation. Optionally, in the step c), the heavy phase obtained from step b) may be treated sequentially with an alkali and an acid, and separated again into a heavy phase and a light phase. The heavy phase is dried, obtaining the glucan preparation. The present invention further relates to the glucan preparation and mannan preparation produced thereby, and the uses thereof.


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