The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Oct. 01, 2013

Filed:

Nov. 24, 2008
Applicants:

Ramkumar Mandalam, Union City, CA (US);

Saadia Faouzi, Daly City, CA (US);

Isabelle Nadeau, San Francisco, CA (US);

Kristina Pfendler-bonham, South San Francisco, CA (US);

Namitha Rao, San Jose, CA (US);

Melissa K. Carpenter, London, CA;

Lakshmi Rambhatla, Redwood City, CA (US);

Choy-pik Chiu, Cupertino, CA (US);

Inventors:

Ramkumar Mandalam, Union City, CA (US);

Saadia Faouzi, Daly City, CA (US);

Isabelle Nadeau, San Francisco, CA (US);

Kristina Pfendler-Bonham, South San Francisco, CA (US);

Namitha Rao, San Jose, CA (US);

Melissa K. Carpenter, London, CA;

Lakshmi Rambhatla, Redwood City, CA (US);

Choy-Pik Chiu, Cupertino, CA (US);

Assignee:

Geron Corporation, Menlo Park, CA (US);

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12N 5/00 (2006.01); C12N 5/10 (2006.01); C12N 5/071 (2010.01); A61K 49/00 (2006.01);
U.S. Cl.
CPC ...
Abstract

This disclosure provides a newly developed strategy and particular options for differentiating pluripotent stem cells into cells of the hepatocyte lineage. Many of the protocols are based on a strategy in which the cells are first differentiated into early germ layer cells, then into hepatocyte precursors, and then into mature cells. The cells obtained have morphological features and phenotypic markers characteristic of human adult hepatocytes. They also show evidence of cytochrome p450 enzyme activity, validating their utility for commercial applications such as drug screening, or use in the manufacture of medicaments and medical devices for clinical therapy.


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