The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Sep. 10, 2013
Filed:
Oct. 14, 2005
Wolf B. Frommer, Washington, DC (US);
Sakiko Okumoto, Washington, DC (US);
Loren Looger, Washington, DC (US);
Marcus Fehr, Washington, DC (US);
Wolf B. Frommer, Washington, DC (US);
Sakiko Okumoto, Washington, DC (US);
Loren Looger, Washington, DC (US);
Marcus Fehr, Washington, DC (US);
Carnegie Institution of Washington, Washington, DC (US);
Abstract
Intramolecular biosensors are disclosed, including PBP-based biosensors, comprising a ligand binding domain fused to donor and fluorescent moieties that permit detection and measurement of Fluorescence Resonance Energy Transfer upon binding ligand. At least one of the donor and fluorescent moieties may be internally fused to the biosensor such that both ends of the internally fused fluorophore are fixed. In addition, methods of improving the sensitivity of terminally fused biosensors are provided. The biosensors of the invention are useful for the detection and quantification of ligands in vivo and in culture.