The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Mar. 05, 2013
Filed:
Apr. 29, 2008
Sidney N Wolfe, Lafayette, CA (US);
Bret a Shirley, Waltham, MA (US);
Susan Babuka, Oakland, CA (US);
Dennis Fordham, Richmond, CA (US);
Irina Esikova, Fremont, CA (US);
Sidney N Wolfe, Lafayette, CA (US);
Bret A Shirley, Waltham, MA (US);
Susan Babuka, Oakland, CA (US);
Dennis Fordham, Richmond, CA (US);
Irina Esikova, Fremont, CA (US);
Novartis Vaccines and Diagnostics, Inc., Emeryville, CA (US);
Abstract
Improved methods for purification and recovery of interferon-beta (IFN-β) and compositions comprising substantially monomeric IFN-β are provided. In one purification method, substantially purified IFN-β or variant thereof is precipitated and then dissolved in a guanidine hydrochloride (HCl) solution. Renaturation of the protein occurs by dilution with a suitable buffer. A similar purification method absent the precipitation step is also provided. Following renaturation of the IFN-β, residual guanidine HCl is removed by diafiltration or dialysis with a pharmaceutically acceptable buffer to prepare pharmaceutical compositions comprising substantially monomeric IFN-β.