The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Oct. 16, 2012
Filed:
Dec. 03, 2009
Timothy J. O'leary, Washington, DC (US);
Jeffrey T. Mason, Rockville, MD (US);
Carol B. Fowler, Rockville, MD (US);
Robert E. Cunningham, Rockville, MD (US);
Timothy J. O'Leary, Washington, DC (US);
Jeffrey T. Mason, Rockville, MD (US);
Carol B. Fowler, Rockville, MD (US);
Robert E. Cunningham, Rockville, MD (US);
The United States of America as Represented by the Department of Veterans Affairs, Washington, DC (US);
The United States of America as Represented by the Secretary of Defense on behalf of the Armed Forces Institute of Pathology, Washington, DC (US);
American Registry of Pathology, Washington, DC (US);
Abstract
A method is disclosed for reversing fixation-induced cross-linking in tissue specimens that have been preserved for histological examination. The method involves placing the fixed tissue in a liquid under elevated temperature and pressure conditions that are sufficient to reverse the fixation-induced cross-linking, restore antigenicity to proteins, and permit improved molecular and proteomic analysis of the preserved tissue specimen. Methods are also disclosed for processing tissues for histological examination under elevated pressure conditions that enhance the perfusion of liquid reagents into the tissue and reduce overall processing times.