The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Aug. 07, 2012
Filed:
Jul. 17, 2008
Joseph Michael Fox, Wilmington, DE (US);
Melissa Blackman, Elkton, MD (US);
Maksim Royzen, Philadelphia, PA (US);
NI Yan, Glen Mills, PA (US);
Joseph Michael Fox, Wilmington, DE (US);
Melissa Blackman, Elkton, MD (US);
Maksim Royzen, Philadelphia, PA (US);
Ni Yan, Glen Mills, PA (US);
University of Delaware, Newark, DE (US);
Abstract
Coupling reactions, suitable for use in organic or aqueous media, are performed by contacting a 1,2,4,5-tetrazine with a dienophile. The dienophile may be covalently bonded to a protein, and the coupling reaction may be performed in biological media such as those containing cells or cell lysates. The reactions may be performed in the presence of primary amines, thiols, acetylenes, azides, phosphines, and products of Staudinger and/or Sharpless-Huisgen reactions Novel 3-substituted cyclopropene compounds and trans-cyclooctenes are exemplary dienophiles for these reactions.