The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Apr. 03, 2012
Filed:
Mar. 17, 2009
Anurag S. Rathore, Thousand Oaks, CA (US);
Stephen B. Lyle, Marcellus, MI (US);
David E. Steinmeyer, Clarkson Valley, MO (US);
Scott I. Allen, St. Peters, MO (US);
John Meyer, Ellisville, MO (US);
Denis M. Boyle, Marthasville, MO (US);
John J. Buckley, Ofallon, MO (US);
Gary V. Johnson, St. Charles, MO (US);
Anurag S. Rathore, Thousand Oaks, CA (US);
Stephen B. Lyle, Marcellus, MI (US);
David E. Steinmeyer, Clarkson Valley, MO (US);
Scott I. Allen, St. Peters, MO (US);
John Meyer, Ellisville, MO (US);
Denis M. Boyle, Marthasville, MO (US);
John J. Buckley, Ofallon, MO (US);
Gary V. Johnson, St. Charles, MO (US);
Pfizer Inc., New York, NY (US);
Abstract
The present invention is directed generally to recombinant methods for making a desired polypeptide. These method(s) yield a polypeptide product containing reduced levels of isoform impurities thereof. In particular, the present invention is directed to (1) a recombinant method for preparing growth hormone with reduced isoform impurities thereof and (2) a recombinant method for preparing a growth hormone antagonist, such as pegvisomant, and its protein intermediate, also having reduced isoform impurities thereof. More specifically, the isoform impurities that are decreased by methods of the present invention are the trisulfide and des-phe isoform impurities of growth hormone and growth hormone antagonist (or its intermediate), respectively.