The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Feb. 28, 2012
Filed:
Aug. 13, 2010
Scott M Kee, Bourbonnais, IL (US);
Paul I. Cook, Kankakee, IL (US);
James R Smith, Bourbonnais, IL (US);
Robert Kling, Bourbonnais, IL (US);
Scott a Fowler, Apex, NC (US);
David Weber, Bourbonnais, IL (US);
Scott M Kee, Bourbonnais, IL (US);
Paul I. Cook, Kankakee, IL (US);
James R Smith, Bourbonnais, IL (US);
Robert Kling, Bourbonnais, IL (US);
Scott A Fowler, Apex, NC (US);
David Weber, Bourbonnais, IL (US);
CSL Behring L.L.C., King of Prussia, PA (US);
Abstract
A streamlined method for purifying alpha-1-antitrypsin (AAT) from an AAT-containing protein mixture, such as a Cohn fraction IV precipitate, is provided. In the method of the invention, contaminating proteins are destabilized by cleavage of disulfide bonds with a reducing reagent, such as a dithiol, which does not affect AAT. The destabilized proteins are then preferentially adsorbed on a solid protein-adsorbing material, without the addition of a salt as a precipitant. Separation of the solid adsorbent from the solution leaves a purified AAT solution that is directly suitable for chromatographic purification, without the need for extensive desalting as in prior art processes. A process incorporating this method, which provides pharmaceutical grade AAT in high yield on a commercial scale, is also described.