The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Aug. 23, 2011
Filed:
Nov. 09, 2006
Gudula Riemen, Langenfeld, DE;
Elke Lorbach, Cologne, DE;
Juliana Helfrich, Glauchau, DE;
Gregor Siebenkotten, Frechen-Koenigsdorf, DE;
Herbert Mueller-hartmann, Cologne, DE;
Kirsten Rothmann-cosic, Berlin, DE;
Corinna Thiel, Köln, DE;
Meike Weigel, Köln, DE;
Heike Wessendorf, Köln, DE;
Helmut Brosterhus, Kirchhundern, DE;
Michael Nix, Köln, DE;
Gudula Riemen, Langenfeld, DE;
Elke Lorbach, Cologne, DE;
Juliana Helfrich, Glauchau, DE;
Gregor Siebenkotten, Frechen-Koenigsdorf, DE;
Herbert Mueller-Hartmann, Cologne, DE;
Kirsten Rothmann-Cosic, Berlin, DE;
Corinna Thiel, Köln, DE;
Meike Weigel, Köln, DE;
Heike Wessendorf, Köln, DE;
Helmut Brosterhus, Kirchhundern, DE;
Michael Nix, Köln, DE;
Lonza Cologne GmbH, Cologne, DE;
Abstract
A method for introducing biologically active molecules into animal or human cells using electric current includes suspending the cells and dissolving the biologically active molecules in a buffer solution which has a buffer capacity of at least 20 mmol×l×pHand an ionic strength of at least 200 mmol×lat a change in the pH from pH 7 to pH 8 and at a temperature of 25° C. to form a suspension. The method further includes applying an electric voltage to the suspension so as to introduce the biologically active molecules into animal or human cells.