The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jun. 28, 2011

Filed:

May. 31, 2005
Applicants:

Madaiah Puttaraju, Germantown, MD (US);

Edward Otto, Reston, VA (US);

Mariano A. Garcia-blanco, Durham, NC (US);

Gerard J. Mcgarrity, Gaithersburg, MD (US);

Gary F. Temple, Washington Grove, MD (US);

Inventors:

Madaiah Puttaraju, Germantown, MD (US);

Edward Otto, Reston, VA (US);

Mariano A. Garcia-Blanco, Durham, NC (US);

Gerard J. McGarrity, Gaithersburg, MD (US);

Gary F. Temple, Washington Grove, MD (US);

Assignee:

VIRxSYS Corporation, Gaitherburg, MD (US);

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12N 5/00 (2006.01); C12N 15/12 (2006.01); C12N 15/63 (2006.01); C12N 15/87 (2006.01);
U.S. Cl.
CPC ...
Abstract

Methods and compositions for generating novel nucleic acid molecules through targeted spliceosome mediated RNA trans-splicing that result in expression of a apoAI protein, an apoAI variant, the preferred embodiment referred to herein as the apoAI Milano variant, a pre-pro-apoAI or an analogue of apoAI. The methods and compositions include pre-trans-splicing molecules (PTMs) designed to interact with a target precursor messenger RNA molecule (target pre-mRNA) and mediate a trans-splicing reaction resulting in the generation of a novel chimeric RNA molecule (chimeric RNA) capable of encoding apoAI, the apoAI Milano variant, or an analogue of apoAI. The expression of this apoAI protein results in protection against vascular disorders resulting from plaque build up, i.e., atherosclerosis, strokes and heart attacks.


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