The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jun. 28, 2011
Filed:
Aug. 21, 2006
Brian T. Chait, New York, NY (US);
Alan J. Tackett, New York, NY (US);
Jeffrey A. Degrasse, New York, NY (US);
Marlene Oeffinger, New York, NY (US);
Michael P. Rout, New York, NY (US);
Matthew D. Sekedat, New York, NY (US);
Brian T. Chait, New York, NY (US);
Alan J. Tackett, New York, NY (US);
Jeffrey A. DeGrasse, New York, NY (US);
Marlene Oeffinger, New York, NY (US);
Michael P. Rout, New York, NY (US);
Matthew D. Sekedat, New York, NY (US);
The Rockefeller University, New York, NY (US);
Abstract
The present invention is method of determining whether or not associations between a given protein and other proteins in a cell are specific. The method comprises (a) providing a first sample of the cells in which the given protein contains a tag, (b) providing a second sample of the same cells, wherein the given protein and the other proteins are metabolically labeled, and wherein neither the given protein nor the other proteins are tagged, (c) mixing and lysing the first cell sample and the second cell sample to provide a mixture of proteins, (d) binding the tag of the given protein to a substrate, (e) isolating proteins associated with the tagged given protein bound to the substrate, whereby the associated proteins comprise: (i) proteins specifically associated with the tagged given protein, (ii) proteins non-specifically associated with the tagged given protein, or (iii) a combination thereof, (f) determining whether each associated protein is unlabeled or a mixture of labeled and unlabeled proteins, wherein if the associated protein is not labeled, then that protein was specifically associated in the cell with the tagged given protein.