The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Dec. 01, 2009
Filed:
May. 23, 2005
Stephen William Watson Michnick, Montreal, CA;
Joelle N Pelletier, Montreal, CA;
Katja M. Arndt, Freiburg, DE;
Andreas Pluckthun, Zürich, CH;
Stephen William Watson Michnick, Montreal, CA;
Joelle N Pelletier, Montreal, CA;
Katja M. Arndt, Freiburg, DE;
Andreas Pluckthun, Zürich, CH;
Odyssey Thera, Inc., San Ramon, CA (US);
Abstract
The present invention describes a rapid and efficient in vivo library-versus-library screening strategy for identifying optimally interacting pairs of heterodimerizing polypeptides. It allows for the screening of a protein library against a second protein library and therefore finds numerous applications in the study of protein-protein interactions. Two leucine zipper libraries, semi-randomized at the positions adjacent to the hydrophobic core, were genetically fused to either one of two designed fragments of the enzyme murine dihydrofolate reductase (mDHFR), and cotransformed into. Interaction between the library polypeptides was required for reconstitution of the enzymatic activity of mDHFR, allowing bacterial growth. Using more weakly associating mDHFR fragments, we increased the stringency of selection. We applied these selection processes to a library-versus-library sample of 2.0×10combinations, and selected a novel leucine zipper pair which may be appropriate for use in further in vivo heterodimerization strategies.