The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Oct. 20, 2009
Filed:
Apr. 12, 2007
Yong Yao, North Potomac, MD (US);
Liang Cao, Bethesda, MD (US);
Jianming LU, Potomac, MD (US);
Isabel Llorente, Adamstown, MD (US);
Yong Yao, North Potomac, MD (US);
Liang Cao, Bethesda, MD (US);
Jianming Lu, Potomac, MD (US);
Isabel Llorente, Adamstown, MD (US);
Atto BioScience Inc., Rockville, MD (US);
Abstract
Disclosed are novel compositions and methods employing cyclic nucleotide-gated channels and fluorescence dyes and other indicators in detecting changes of intracellular cAMP levels, for instance in response to the stimulation of G-protein-coupled receptors. CNG mutants comprising one or more pore mutations that enhance sensitivity to cAMP and decrease divalent cation-mediated blockage are used in a variety of assays, including detection of the G-protein-coupled receptor activity. Also disclosed are novel dye quencher formulations that are particularly suited for cell-based assays of protein interactions.