The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Oct. 13, 2009

Filed:

Jun. 06, 2006
Applicants:

Jan Berka, Guilford, CT (US);

Zhoutao Chen, Milford, CT (US);

Michael Egholm, Woodbridge, CT (US);

Brian C. Godwin, North Haven, CT (US);

Stephen Kyle Hutchison, Branford, CT (US);

John Harris Leamon, Guilford, CT (US);

Gary James Sarkis, Guilford, CT (US);

Jan Fredrik Simons, Guilford, CT (US);

Inventors:

Jan Berka, Guilford, CT (US);

Zhoutao Chen, Milford, CT (US);

Michael Egholm, Woodbridge, CT (US);

Brian C. Godwin, North Haven, CT (US);

Stephen Kyle Hutchison, Branford, CT (US);

John Harris Leamon, Guilford, CT (US);

Gary James Sarkis, Guilford, CT (US);

Jan Fredrik Simons, Guilford, CT (US);

Assignee:

454 Life Sciences Corporation, Branford, CT (US);

Attorneys:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12Q 1/68 (2006.01); C12P 19/34 (2006.01); C07H 21/04 (2006.01);
U.S. Cl.
CPC ...
Abstract

The present invention provides for a method of preparing a target nucleic acid fragments to produce a smaller nucleic acid which comprises the two ends of the target nucleic acid. Specifically, the invention provides cloning and DNA manipulation strategies to isolate the two ends of a large target nucleic acid into a single small DNA construct for rapid cloning, sequencing, or amplification.


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