The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Sep. 15, 2009

Filed:

May. 21, 2004
Applicants:

Takeshi Matsuoka, Shizuoka, JP;

Shinji Koga, Shizuoka, JP;

Takuji Kouzuma, Shizuoka, JP;

Issei Yoshioka, Shizuoka, JP;

Atsuhisa Nishimura, Aichi, JP;

Homare Itou, Aichi, JP;

Toshihiko Kumazawa, Aichi, JP;

Takashi Kuroyanagi, Aichi, JP;

Inventors:

Takeshi Matsuoka, Shizuoka, JP;

Shinji Koga, Shizuoka, JP;

Takuji Kouzuma, Shizuoka, JP;

Issei Yoshioka, Shizuoka, JP;

Atsuhisa Nishimura, Aichi, JP;

Homare Itou, Aichi, JP;

Toshihiko Kumazawa, Aichi, JP;

Takashi Kuroyanagi, Aichi, JP;

Assignees:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/26 (2006.01); C12Q 1/37 (2006.01);
U.S. Cl.
CPC ...
Abstract

There is provided a method for specifically determining a glycated β-chain N-terminal of glycated hemoglobin using enzymes without a separation operation, and a determination reagent kit therefor. A protease that cleaves a glycated amino acid and/or a glycated peptide from a glycated β-chain N-terminal without substantially cleaving a glycated amino acid or a glycated peptide from a glycated α-chain N-terminal of glycated hemoglobin or a fragment thereof is screened. The method of specifically determining a glycated β-chain N-terminal of glycated hemoglobin and the determination reagent kit are provided by using the protease obtained by the screening method. According to the present invention, a glycated β-chain N-terminal of glycated hemoglobin can specifically be determined without a separation operation.


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