The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jun. 09, 2009
Filed:
May. 23, 2003
Christopher William Pugh, Oxford, GB;
Jonathan Mark Gleadle, Oxford, GB;
Peter John Ratcliffe, Oxford, GB;
Christopher Joseph Schofield, Oxford, GB;
Luke Alexander Mcneill, Oxford, GB;
Christopher William Pugh, Oxford, GB;
Jonathan Mark Gleadle, Oxford, GB;
Peter John Ratcliffe, Oxford, GB;
Christopher Joseph Schofield, Oxford, GB;
Luke Alexander McNeill, Oxford, GB;
Isis Innovation Limited, Oxford, GB;
Abstract
A method of identifying an agent which modulates hydroxylation of hypoxia inducible factor (HIF), comprises contacting a HIF asparagine hydroxylase and a test substance in the presence of a substrate of the hydroxylase under conditions in which asparagine in the substrate is hydroxylated in the absence of the test substance; and determining hydroxylation of the substrate. Preferably the substrate is a HIF polypeptide comprising HIF-1α, a fragment thereof comprising Asn 803 of HIF-1α or a peptide analogue of HIF-1α or fragment thereof comprising an asparagine equivalent to Asn 803 of HIF-1α and wherein hydroxylation of Asn 803 or of a said equivalent asparagine is determined.