The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Mar. 24, 2009
Filed:
Oct. 04, 2004
Zhenjun Diwu, Sunnyvale, CA (US);
Jesse J. Twu, Cupertino, CA (US);
Guoliang Yi, Sunnyvale, CA (US);
Luke D. Lavis, Sunnyvale, CA (US);
Yen-wen Chen, San Francisco, CA (US);
Kelly J. Cassutt, Somerset, NJ (US);
Zhenjun Diwu, Sunnyvale, CA (US);
Jesse J. Twu, Cupertino, CA (US);
Guoliang Yi, Sunnyvale, CA (US);
Luke D. Lavis, Sunnyvale, CA (US);
Yen-Wen Chen, San Francisco, CA (US);
Kelly J. Cassutt, Somerset, NJ (US);
MDS Analytical Technologies (US) Inc., Sunnyvale, CA (US);
Abstract
Systems, including compositions and methods, for measuring pH, particularly in cells, organelles, and other samples. The compositions include pH-sensitive fluorescent and fluorogenic 2',7′-dialkylfluorescein derivatives and associated nonfluorescent precursor compounds. The compositions may permit ratiometric measurement in the excitation spectrum and the emission spectrum. The methods include adding a precursor compound to a sample cell, incubating the sample cell to release the free indicator, illuminating the sample cell, and detecting the fluorescence response of the free indicator.