The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Dec. 02, 2008
Filed:
Apr. 03, 2001
Patricia C. Weber, Yardley, PA (US);
Paul Reichert, Montville, NJ (US);
Vincent S. Madison, Mountain Lakes, NJ (US);
Daniel F. Wyss, Convent Station, NJ (US);
Nanhua Yao, Irvine, CA (US);
Dingjiang Liu, Edison, NJ (US);
Jennifer J. Gesell, Hamilton Square, NJ (US);
Patricia C. Weber, Yardley, PA (US);
Paul Reichert, Montville, NJ (US);
Vincent S. Madison, Mountain Lakes, NJ (US);
Daniel F. Wyss, Convent Station, NJ (US);
Nanhua Yao, Irvine, CA (US);
Dingjiang Liu, Edison, NJ (US);
Jennifer J. Gesell, Hamilton Square, NJ (US);
Schering Corporation, Kenilworth, NJ (US);
Abstract
This invention provides fragments of HCV NS3 helicase, and crystalline compositions thereof, based on subdomains of HCV helicase protein. The protein fragments are stable, soluble, and structurally sound. They can be expressed at high levels in conventional expressions systems, such as, to permit efficient, large-scale production for NMR-based screening applications and production of [H,C,N]- and [C,N]-labeled polypeptides for structural NMR studies. Helicase fragments of the present invention are useful in the most advanced NMR techniques available, e.g., NMR-based drug discovery techniques such as SAR-by-NMR, in biological assays to discover inhibitors of HCV NS3 helicase, and to evaluate the mechanism of action and substrates for HCV NS3 helicase. Crystals of the present invention are useful for structure-based drug design studies using x-ray crystallographic techniques.