The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Sep. 09, 2008

Filed:

May. 10, 2001
Applicants:

Toshihiro Kuroita, Tsuruga, JP;

Masao Kitabayashi, Tsuruga, JP;

Yoshikazu Ishida, Tsuruga, JP;

Hideyuki Komatsubara, Tsuruga, JP;

Yoshiaki Nishiya, Tsuruga, JP;

Bunsei Kawakami, Tsuruga, JP;

Yoshihisa Kawamura, Tsuruga, JP;

Tadayuki Imanaka, Suita, JP;

Inventors:

Toshihiro Kuroita, Tsuruga, JP;

Masao Kitabayashi, Tsuruga, JP;

Yoshikazu Ishida, Tsuruga, JP;

Hideyuki Komatsubara, Tsuruga, JP;

Yoshiaki Nishiya, Tsuruga, JP;

Bunsei Kawakami, Tsuruga, JP;

Yoshihisa Kawamura, Tsuruga, JP;

Tadayuki Imanaka, Suita, JP;

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 9/12 (2006.01); C12P 19/34 (2006.01);
U.S. Cl.
CPC ...
Abstract

An object of the present invention is to provide a thermostable DNA polymerase with enhanced amplification efficiency and/or improved fidelity in polymerase chain reaction (PCR), and provide a process for production thereof. More specifically, the present invention provides thermostable DNA polymerase wherein in the DXEXXXH sequence (D: aspartic acid, E: glutamic acid, H: histidine, X, X, Xand X: any amino acid) consisting of DXE sequence within the EXO I region and a four amino acid length peptide adjacent to said glutamic acid(E) of thermostable DNA polymerase having 3'-5′ exonuclease activity, histidine(H) has been replaced by another amino acid.


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