The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Feb. 19, 2008
Filed:
Apr. 23, 2002
Gudula Riemen, Langenfeld, DE;
Elke Lorbach, Köln, DE;
Juliana Helfrich, Köln, DE;
Gregor Siebenkotten, Frechen-Königsdorf, DE;
Herbert Müller-hartmann, Köln, DE;
Kirsten Rothmann, Köln, DE;
Corinna Thiel, Köln, DE;
Meike Weigel, Köln, DE;
Heike Wessendorf, Köln, DE;
Helmut Brosterbus, Kirchhundern, DE;
Michael Nix, Köln, DE;
Gudula Riemen, Langenfeld, DE;
Elke Lorbach, Köln, DE;
Juliana Helfrich, Köln, DE;
Gregor Siebenkotten, Frechen-Königsdorf, DE;
Herbert Müller-Hartmann, Köln, DE;
Kirsten Rothmann, Köln, DE;
Corinna Thiel, Köln, DE;
Meike Weigel, Köln, DE;
Heike Wessendorf, Köln, DE;
Helmut Brosterbus, Kirchhundern, DE;
Michael Nix, Köln, DE;
Amaxa AG, , DE;
Abstract
The invention relates to a buffer solution for suspending animal or human cells and for dissolving biologically active molecules in order to introduce biologically active molecules into cells using an electric current. The inventive buffer solution has a buffering capacity of at least 20 mmol×pHand an ionic strength of at least 200 mmol×1during a change to the pH value from pH 7 to pH 8 and at a temperature of 25° C. The use of a buffer solution of this type allows biologically active molecules to be introduced into animal and human cells with a high degree of transfection efficiency and at the same time a low cell mortality. Different cell types, in particular dormant and actively dividing cells of low activity, can be successfully transfected in the buffer solution.