The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jan. 08, 2008
Filed:
May. 24, 2005
William R. Davidson, Thornhill, Ontario, CA;
Bori Shushan, Toronto, Ontario, CA;
Peter Kovarik, Markham, Ontario, CA;
Tom R. Covey, Dundas, Ontario, CA;
John D. Brennan, Dundas, Ontario, CA;
William R. Davidson, Thornhill, Ontario, CA;
Bori Shushan, Toronto, Ontario, CA;
Peter Kovarik, Markham, Ontario, CA;
Tom R. Covey, Dundas, Ontario, CA;
John D. Brennan, Dundas, Ontario, CA;
Other;
Abstract
Sol-gel derived monolithic silica columns containing entrapped dihydrofolate reductase were used for frontal affinity chromatography of small molecule mixtures. The output from the column combined with a second stream containing the matrix molecule (HCCA) and was directly deposited onto a conventional MALDI plate that moved relative to the column via a computer controlled x-y stage, creating a semi-permanent record of the FAC run. The use of MALDI MS allowed for a decoupling of the FAC and MS methods allowing significantly higher ionic strength buffers to be used for FAC studies, which allowed for better retention of protein activity over multiple runs.