The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Aug. 14, 2007
Filed:
Jul. 09, 2004
Claudio Dalvit, Milan, IT;
Elena Ardini, Milan, IT;
Maria Magdalena Flocco, Milan, IT;
Gianpaolo Fogliatto, Varese, IT;
Nicola Mongelli, Milan, IT;
Marina Veronesi, Milan, IT;
Claudio Dalvit, Milan, IT;
Elena Ardini, Milan, IT;
Maria Magdalena Flocco, Milan, IT;
Gianpaolo Fogliatto, Varese, IT;
Nicola Mongelli, Milan, IT;
Marina Veronesi, Milan, IT;
Pfizer Italia S.r.l., Rome, IT;
Abstract
High-Throughput Screening (HTS) of large compound libraries is the method of drug-lead discovery. It is now well accepted that for a functional assay, quality is more important than quantity. A biochemical NMR method originally proposed by Percival and Withers (Biochemistry, 1992, 31, 498–505) is extended to the screening of Ser/Thr kinases. The method requires the presence of a CF(or CF) moiety on the substrate and utilizesF NMR spectroscopy for the detection of the starting and enzymatically modified substrates. Experiments can be performed in real time or in an endpoint assay format using protein and substrate concentrations comparable to the ones used by other HTS techniques. Application of this technique to the phosphorylation of a substrate by the protein Ser/Thr kinase AKT1 is presented.