The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jul. 03, 2007
Filed:
Jul. 28, 2004
Suzanne L. Bolten, Kirkwood, MO (US);
Robert A. Clayton, Foristell, MO (US);
Alan M. Easton, Maryland Heights, MO (US);
Leslie C. Engel, Des Peres, MO (US);
Dean M. Messing, St. Louis, MO (US);
John S. NG, Thousand Oaks, CA (US);
Beverly Reitz, Chesterfield, MO (US);
Mark C. Walker, Chesterfield, MO (US);
Ping T. Wang, Chesterfield, MO (US);
Suzanne L. Bolten, Kirkwood, MO (US);
Robert A. Clayton, Foristell, MO (US);
Alan M. Easton, Maryland Heights, MO (US);
Leslie C. Engel, Des Peres, MO (US);
Dean M. Messing, St. Louis, MO (US);
John S. Ng, Thousand Oaks, CA (US);
Beverly Reitz, Chesterfield, MO (US);
Mark C. Walker, Chesterfield, MO (US);
Ping T. Wang, Chesterfield, MO (US);
Pharmacia Corporation, St. Louis, MO (US);
Abstract
The present invention relates to a novel cytochrome P450-like enzyme (11 alpha hydroxylase) and an oxidoreductase (oxidoreductase) isolated from cDNA library generated from the mRNA ofspores. When the cDNA encoding the 11 alpha hydroxylase was co-expressed in(Sf-9) insect cells with the cDNA encoding human oxidoreductase as an electron donor, it successfully catalyzed the conversion of the steroid substrate 4-androstene-3,17-dione (AD) to 11 alpha-hydroxy-AD as determined by HPLC analysis. The invention also relates to nucleic acid molecules associated with or derived from these cDNAs including complements, homologues and fragments thereof, and methods of using these nucleic acid molecules, to generate, for example, polypeptides and fragments thereof. The invention also relates to the generation of antibodies that recognizes the11 alpha hydroxylase and oxidoreductase and methods of using these antibodies to detect the presence of these native and recombinant polypeptides within unmodified and transformed host cells, respectively. The invention also provides methods of expressing the11 alpha hydroxylase gene separately, or in combination with human oroxidoreductase, in heterologous host cells, to facilitate the bioconversion of steroid substrates to their 11 alpha hydroxy-counterparts.