The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jul. 18, 2006

Filed:

Dec. 30, 1999
Applicants:

Rex M. Bitner, Cedarburg, WI (US);

Craig E. Smith, Oregon, WI (US);

Douglas H. White, Madison, WI (US);

Braeden L. Butler, Madison, WI (US);

Jacqui Sankbeil, Edgerton, WI (US);

Inventors:

Rex M. Bitner, Cedarburg, WI (US);

Craig E. Smith, Oregon, WI (US);

Douglas H. White, Madison, WI (US);

Braeden L. Butler, Madison, WI (US);

Jacqui Sankbeil, Edgerton, WI (US);

Assignee:

Promega Corporation, Madison, WI (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12K 1/08 (2006.01); C12Q 1/68 (2006.01); C12M 1/34 (2006.01); G01N 33/566 (2006.01); C07H 21/02 (2006.01);
U.S. Cl.
CPC ...
Abstract

Methods are disclosed for using paramagnetic particles to concentrate or harvest cells. Methods are also disclosed for clearing a solution of disrupted biological material, such as a lysate of cells or a homogenate of mammalian tissue. Methods are also disclosed for using paramagnetic particles to isolate target nucleic acids, such as RNA or DNA, from a solution cleared of disrupted biological material using the same type or a different type of paramagnetic particle. Kits are also disclosed for use with the various methods of the present invention. Nucleic acids isolated according to the present methods and using the present kits are suitable for immediate use in downstream processing, without further purification.


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