The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
May. 11, 2004
Filed:
Feb. 22, 2001
Jonathan Schneck, Silver Spring, MD (US);
Drew Pardoll, Brookeville, MD (US);
Sean O'Herrin, Baltimore, MD (US);
Jill Slansky, Baltimore, MD (US);
Tim Greten, Baltimore, MD (US);
The Johns Hopkins University, Baltimore, MD (US);
Abstract
To increase the effective affinity of soluble analogs of peptide/MHC molecules for their cognate ligands, divalent peptide/MHC complexes were constructed. Using a recombinant DNA strategy, DNA encoding the MHC class I was ligated to DNA coding for murine Ig heavy chain. MHC/Ig complexes were exploited to homogeneously load with peptides of interest. The results of flow cytometry demonstrated that the MHC/Ig complexes bound specifically with high affinity to cells bearing their cognate receptors. MHC/Ig complexes are also useful in modulating effector functions of antigen-specific T cells. These MHC/Ig complexes are useful for studying TCR/MHC interactions and lymphocyte tracking and have uses as specific regulators of immune responses.