The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
May. 07, 2002
Filed:
Oct. 14, 1999
Susana Vazquez Ramudo, Habana, CU;
Guadalupe Guzman Tirado, Habana, CU;
Gerardo Enrique Guillen Nieto, Habana, CU;
Orlando Luis Pardo Lazo, Habana, CU;
Glay Chinea Santiago, Habana, CU;
Ana Beatriz Perez Diaz, Habana, CU;
Maritza Pupo Antunez, Habana, CU;
Rosmari Rodriguez Roche, Habana, CU;
Osvaldo Reyes Acosta, Habana, CU;
Hilda Elisa Garay Perez, Habana, CU;
Gabriel Padron Palomares, Habana, CU;
Maylin Alvarez Vera, Habana, CU;
Luis Morier Diaz, Habana, CU;
Omaida Perez Insuita, Habana, CU;
Jose Luis Pelegrino Martinez De La Cotera, Habana, CU;
Other;
Abstract
The present invention relates to five synthetic peptides of pre-M/M protein of Dengue-2 virus, corresponding to amino acid sequences 3-31, 45-67, 57-92, 69-93 and 103-124. The anti-peptide immune response was evaluated in mice. Recombinant fusion proteins were also obtained, including regions of pre-M/M protein. The presence of B cell epitopes in both mice and humans was demonstrated in the pre-M/M protein peptides. Peptides 3-31 and 103-124 elicited neutralizing antibodies against the four serotypes of Dengue virus. Virus-specific proliferative responses were demonstrated in mice immunized with non-conjugated peptides 3-31 and 57-92. Mice immunized with conjugated peptides 3-31, 57-92, and 69-93 were protected when they were challenged with Dengue-2 virus. Thus, the presence of sequential epitopes in Pre-M/M protein of Dengue-2 virus was demonstrated, as well as their relevance in the immune response against this flavivirus.