The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Apr. 30, 2002

Filed:

Aug. 26, 1999
Applicant:
Inventors:

Nanibhushan Dattagupta, San Diego, CA (US);

C. Nagaraja Sridhar, San Diego, CA (US);

Whei-Kuo Wu, San Diego, CA (US);

Assignee:

Applied Gene Technologies, Inc., San Diego, CA (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12P 1/934 ; C12Q 1/68 ; C07H 1/900 ; C07H 2/104 ; C07H 2/102 ;
U.S. Cl.
CPC ...
C12P 1/934 ; C12Q 1/68 ; C07H 1/900 ; C07H 2/104 ; C07H 2/102 ;
Abstract

The present invention provides a cocktail of reagents for nucleic acid amplification that are stabilized by inclusion of a reversible inhibitor of undesirable reactions. Such cocktail of reagents eliminates the requirement for separate preparation and quality control of each reagent used in a reaction. Methods to prepare stabilized cocktails and to use stabilized cocktails also are included. The stabilized cocktail compositions also can include reagents to release nucleic acid from cells and to label the nucleic acid, allowing detection of nucleic acid in a sample with a single reagent addition step. The invention also provides kits for performing the above methods.


Find Patent Forward Citations

Loading…