The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Nov. 20, 2001
Filed:
May. 06, 1993
Melvyn Little, Neckargemünd, DE;
Frank Berthold Breitling, Wiesloch, DE;
Thomas Seehaus, Heppenheim, DE;
Stefan Dübel, Heidelberg, DE;
Iris Klewinghaus, Mannheim, DE;
Dade Behring Marburg GmbH, Marburg, DE;
Abstract
The invention relates to the preparation and use of gene banks of human antibodies (Ab). Starting from a mixture of human B-lymphocytes, their mRNA is translated into the cDNA using oligo-dT primers. Subsequently, an amplification of the Ab-specific cDNA by means of polymerase chain reaction (PCR) takes place using suitable oligonucleotide primer sequences. Expression of this amplified Ab-specific cDNA in a bacterial expression vector, e.g. the vector pFMT described below, in,thus makes available a human-antibody library with a comprehensive repertoire for screening selected antigens in vitro.