The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Apr. 03, 2001
Filed:
Jul. 06, 1999
Applicant:
Inventors:
Assignee:
Pyrosequencing AB, Uppsala, SE;
Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12Q 1/68 ; C12Q 1/66 ; C12P 1/934 ; C07H 2/104 ;
U.S. Cl.
CPC ...
C12Q 1/68 ; C12Q 1/66 ; C12P 1/934 ; C07H 2/104 ;
Abstract
The present invention provides a method of identifying a base at a target position in a single-stranded sample DNA sequence wherein an extension primer, which hybridizes to the sample DNA immediately adjacent to the target position, is provided and the sample DNA and extension primer are subjected to a polymerization reaction in the presence of a deoxynucleotide or dideoxynucleotide, whereby the deoxynucleotide or dideoxynucleotide will only become incorporated and release pyrophosphate if it is complementary to the base in the target position. Release of pyrophosphate is detected enzymatically and pyrophosphate detection enzyme(s) are included in the polymerization step.
Published as:
GB9620209D0; WO9813523A1; AU4391897A; EP0932700A1; JP2001501092A; US6210891B1; EP0932700B1; DE69706430D1; DE69706430T2; JP3510272B2;