The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Nov. 28, 2000

Filed:

Jul. 23, 1993
Applicant:
Inventors:

Joseph Y Tai, Fort Washington, PA (US);

Jeffrey K Pullen, Columbia, MD (US);

Thomas Soper, Laurel, MD (US);

Shu-Mei Liang, Taipei, TW;

Milan S Blake, Fulton, MD (US);

Assignee:

North American Vaccine, Inc., Columbia, MD (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N / ; C12N / ; C12P / ; A61K / ;
U.S. Cl.
CPC ...
435 693 ; 4242561 ; 435 697 ; 4352528 ; 43525233 ; 4351723 ;
Abstract

The present invention relates, in general, to a method of expressing the outer membrane protein P2 from Haemophilus influenzae type b (Hib-P2) and fusion proteins thereof. In particular, the present invention relates to a method of expressing the outer membrane protein P2 from Haemophilus influenzae type b or fusion protein thereof in E. coli wherein the Hib-P2 protein or fusion protein comprises more than 2% of the total protein expressed in E. coli. The invention also relates to a method of purification and refolding of Hib-P2 protein and fusion protein thereof.


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