The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Apr. 20, 1999
Filed:
Sep. 25, 1996
Barbara A Chandler, Lexington, MA (US);
Kermit M Borland, Shrewsbury, MA (US);
Shawn P Cain, North Chelmsford, MA (US);
Claudy J-P Mullon, Framingham, MA (US);
W.R. Grace & Co.-Conn., Lexington, MA (US);
Abstract
The invention features a method of processing cryopreserved cells by thawing and equilibrating the cells at warm temperatures (e.g., between 30.degree. C. and 43.degree. C). Either the cell suspension in the cryoprotective medium is thawed to a temperature between 35.degree. C. and 43.degree. C. or the cryoprotective medium is equilibrated with a culture medium at a temperature between 35.degree. C. and 43.degree. C., or both steps are carried out at the warm temperatures. By thawing and equilibrating the cryopreserved cells at warm temperatures, the viability, (especially after 3 hours of culture), and metabolic activity (i.e., diazepam metabolism) of the cells can be improved over traditional cold cell processing (i.e., at temperatures of between 2.degree. C. and 8.degree. C.).