The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Feb. 03, 1998
Filed:
Mar. 20, 1996
Applicant:
Inventors:
Peter Michael Hammond, Salisbury, GB;
Graham Mark Brearley, 1000 Oaks, CA (US);
Christopher Philip Price, Stapleford, GB;
Assignee:
Microbiological Research Authority, Salisbury, GB;
Primary Examiner:
Int. Cl.
CPC ...
C12N / ; C12P / ;
U.S. Cl.
CPC ...
435191 ; 435108 ; 435189 ; 4352532 ; 435872 ;
Abstract
A method for producing in culture phenylalanine dehydrogenase at yields of 50+ units of enzyme per litre of culture, one unit measured as the amount required to convert one micro-mole of phenylalanine to phenyl pyrovic acid at 25.degree. C. and pH 10.8, in a growth medium comprising a nitrogen source, a buffer, phenylalanine or phenylpyruvic acid or a salt thereof at 20 mM and optionally a vitamin solution. Using phenylalanine at about 100 mM gives yields of phenylalanine dehydrogenase enzyme of 350+ units per liter of culture.