The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Aug. 19, 1997

Filed:

Oct. 27, 1994
Applicant:
Inventors:

Stephen T Yue, Eugene, OR (US);

Victoria L Singer, Eugene, OR (US);

Bruce L Roth, Corvallis, OR (US);

Thomas J Mozer, Eugene, OR (US);

Paul J Millard, Eugene, OR (US);

Laurie J Jones, Monroe, OR (US);

Xiaokui Jin, Springfield, OR (US);

Richard P Haugland, Eugene, OR (US);

Assignee:

Molecular Probes, Inc., Eugene, OR (US);

Attorneys:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12Q / ; C12Q / ; G01N / ; C07H / ;
U.S. Cl.
CPC ...
435 34 ; 435-4 ; 435 29 ; 435-6 ; 436 94 ; 436800 ; 536 2673 ; 536-111 ; 536 256 ;
Abstract

The invention describes the preparation and use of fluorescent stains for nucleic acids derived from unsymmetrical cyanine dyes comprising a substituted benzazolium ring system linked by a methine bridge to a pyridinium or quinolinium ring system having at least one substituent on the pyridinium or quinolinium ring that contains a heteroatom. The presence of the heteroatom-containing substituent results in higher sensitivity to oligonucleotides and larger nucleic acid polymers in a wide range of cells and gels, and for use in analysis of cell structure, membrane integrity or function.

Published as:
CA2179284A1; WO9613552A2; AU3967295A; WO9613552A3; EP0740689A1; JPH09507879A; US5658751A; US5863753A; AU714890B2; EP0740689B1; ATE212653T1; DE69525239D1; DE69525239T2; CA2179284C; JP2007291403A; JP2009280820A; JP4503047B2; JP4634514B2;

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