The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jan. 14, 1997
Filed:
Jul. 28, 1994
Applicant:
Inventors:
Pedro Santamaria, Calgary, CA;
Michael T Boyce-Jacino, Finksburg, MD (US);
Jose J Barbosa, Roseville, MN (US);
Stephen S Rich, Mocksville, NC (US);
Anthony J Faras, Long Lake, MN (US);
Assignee:
Regents of the University of Minnesota, , MN (US);
Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12Q / ; C12P / ; C07H / ;
U.S. Cl.
CPC ...
435-6 ; 435 912 ; 536 243 ; 536 2431 ; 536 2433 ;
Abstract
The present invention provides a process for determining genotypes in highly polymorphic systems by polymerase chain reaction amplification of cDNA or genomic DNA and direct sequencing polymerase chain reaction products using oligonucleotide primers. More specifically, Class I HLA genotypes can be unambiguously determined in any subject in 16-24 hours by direct sequencing of HLA-A, HLA-B, and HLA-C transcripts enzymatically amplified and sequenced using a limited number of selected oligonucleotide.