The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Apr. 11, 1995

Filed:

Mar. 07, 1994
Applicant:
Inventors:

James H Jett, Los Alamos, NM (US);

Richard A Keller, Los Alamos, NM (US);

John C Martin, Los Alamos, NM (US);

Richard G Posner, Los Alamos, NM (US);

Babetta L Marrone, Los Alamos, NM (US);

Mark L Hammond, Los Alamos, NM (US);

Daniel J Simpson, Los Alamos, NM (US);

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12Q / ; C12P / ; G01N / ;
U.S. Cl.
CPC ...
435-6 ; 435 911 ; 436 94 ;
Abstract

Method for rapid-base sequencing in DNA and RNA with two-base labeling and employing fluorescent detection of single molecules at two wavelengths. Bases modified to accept fluorescent labels are used to replicate a single DNA or RNA strand to be sequenced. The bases are then sequentially cleaved from the replicated strand, excited with a chosen spectrum of electromagnetic radiation, and the fluorescence from individual, tagged bases detected in the order of cleavage from the strand.


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