The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Feb. 14, 1995

Filed:

Nov. 27, 1990
Applicant:
Inventors:

Andrew J Makoff, Beckenham, GB;

Michael A Romanos, Beckenham, GB;

Jeffrey J Clare, Beckenham, GB;

Neil F Fairweather, Beckenham, GB;

Assignee:

Evans Medical Limited, Leatherhead, GB;

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
A61K / ; C12N / ; C12N / ;
U.S. Cl.
CPC ...
435 693 ; 424 88 ; 4241901 ; 4242611 ; 435 691 ; 4351723 ; 4352523 ; 43525411 ; 4352542 ; 43525421 ; 4353201 ; 536 231 ; 536 237 ;
Abstract

Expression of tetanus toxin fragment C is accomplished employing a DNA coding sequence having a (G+C)-content that has been increased in the region from nucleotide 410 to the 3' end of the coding sequence relative to the wild-type DNA sequence. This allows the production of complete mRNA transcripts. Typically the (G+C)-content is increased in the following regions: (i) nucleotides 510-710, (ii) nucleotides 650-850, (iii) nucleotides 800-1100, (iv) nucleotides 900-1200 and (v) nucleotides 1100 to the 3' end of the coding sequence. The (G+C)-content may also be increased in the region of nucleotides 410-610. These regions in the wild-type DNA encompass terminator sequences.


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