The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jun. 04, 1991

Filed:

Aug. 17, 1989
Applicant:
Inventors:

Salvatore Toma, Milan, IT;

Marina Del Bue, Milan, IT;

Antonio Mele, Pavia, IT;

Guido Grandi, Segrate, IT;

Assignee:

Eniricerche S.p.A., Milan, IT;

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12N / ; C12N / ; C12N / ;
U.S. Cl.
CPC ...
435 691 ; 435 711 ; 435 91 ; 435170 ; 4351721 ; 4351723 ; 43525231 ; 4353201 ; 435839 ; 536 27 ; 935-6 ; 935-8 ; 935-9 ; 935 22 ; 935 29 ; 935 33 ; 935 38 ; 935 59 ; 935 60 ; 935 61 ; 935 74 ;
Abstract

A description is given of a cloning vector useful for the expression and secretion of heterologous proteins in Bacillus subtilis, molecules of recombinant DNA formed by the said cloning vector, expediently cut at a restriction site located within the neutral protease gene, and by the sequence of heterologous DNA which codes for the protein of interest, Bacillus subtilis cells transformed with the said molecules and capable of expressing the sequence of heterologous DNA and of producing and secreting the protein coded by the said sequence of heterologous DNA in high yields. The proteins obtained in this manner are useful in the fields of pharmaceuticals and foods, or as compounds for the production of substances which are normally obtained by chemical synthesis.


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