The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Aug. 18, 1987

Filed:

Jun. 16, 1986
Applicant:
Inventors:

Akihiro Ginnaga, Kumamoto, JP;

Hiroshi Koba, Kumamoto, JP;

Shin Sakuma, Kumamoto, JP;

Hisashi Kitagawa, Kumamoto, JP;

Akira Yamada, Kumamoto, JP;

Yoji Suzuki, Hino, JP;

Assignees:
Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12P / ;
U.S. Cl.
CPC ...
435 68 ; 435-3 ; 435802 ; 435253 ; 435822 ; 435812 ; 435813 ; 424 92 ; 424 88 ; 424 93 ;
Abstract

A method for production of HA fraction containing protective antigens of Bordetella pertussis (i.e. a fraction containing F-HA and LPF-HA) in an industrial scale, for instance, with a fermentater, which comprises inoculating a strain of B. pertussis in a liquid culture containing a cyclodextrin or its derivative, culturing it by a spinner culture under controlling the culture temperature and the amount of dissolved oxygen and under defoaming condition, optionally under controlling the pH range, and harvesting the produced HA fraction from the culture broth at a stage of from logarithmic growth phase to static grow phase, and a method for production of a pertussis vaccine from the HA fraction thus obtained by formalinizing the HA fraction in the presence of an amino acid.


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