The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jun. 16, 1987

Filed:

Apr. 09, 1985
Applicant:
Inventors:

Josephine Grosch, Elkhart, IN (US);

Gary A Wilson, Elkhart, IN (US);

Karen Wollweber, South Bend, IN (US);

Clifford O Yehle, Elkhart, IN (US);

Assignee:

Miles Laboratories, Inc., Elkhart, IN (US);

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12Q / ;
U.S. Cl.
CPC ...
435 34 ; 435 30 ; 435 38 ;
Abstract

A method is provided for detecting a microorganism which produces a desired substance. The method involves overlaying a membrane on an agar surface in a predetermined orientation. Microorganisms are grown on the membrane and the substance allowed to pass into the agar. The membrane is removed and a detectable reaction between the substance and one or more reagents is observed. The location of the microorganism to be detected can be determined by the predetermined orientation, and the microorganism can be isolated by removing it from the membrane. When the substance is not secreted by the microorganism, the microorganisms are lysed releasing the substance for passage into the agar. The microorganisms producing the substance can be located on replicate plates and isolated.


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