The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Apr. 25, 1978
Filed:
Sep. 28, 1976
Lars-Olof Edvard Lindquist, Uppsala, SW;
Jan Hakan Berglof, Uppsala, SW;
John Malcolm Curling, Uppsala, SW;
Pharmacia Fine Chemicals AB, Uppsala, SW;
Abstract
This invention relates to a new method of isolating highly purified albumin in high yields from plasma products. According to the invention a particular plasma fraction, containing albumin in dissolved form and being essentially free from the coagulation factors I, II, VII, VIII, IX and X and from the main part of IgG, is subjected to a two-step chromatographic separation using aqueous buffer systems. The first step is carried out on an anion exchanger and the second step on a cation exchanger, or vice versa. In a preferred embodiment a buffer of pH 4.5 - 4.9 and an ionic strength of 0.025 - 0.1 is used for the separation on the anion exchanger, whereas a buffer of pH 5.2 - 6.5 and an ionic strength of 0.1 - 0.05 is used for the separation on the cation exchanger.