The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Apr. 29, 2025
Filed:
Jan. 31, 2022
Miltenyi Biotec B.v. & Co. KG, Bergisch Gladbach, DE;
Thomas Rothmann, Langenfeld, DE;
Robert Pinard, Lowell, MA (US);
Hansueli Meyer, Acton, MA (US);
Andreas Bosio, Cologne, DE;
Heinrich Spiecker, Bielefeld, DE;
Seiyu Hosono, Stoneham, MA (US);
Ryan Hindman, Bergisch Gladbach, DE;
Emily Neil, Bergisch Gladbach, DE;
Chris Nehme, Bergisch Gladbach, DE;
Miltenyi Biotec B.V. & Co. KG, Bergisch Gladbach, DE;
Abstract
Microscopy imaging that allow for multiple mRNAs, proteins and metabolites to be spatially resolved at a subcellular level provides valuable molecular information which is a crucial factor for understanding tissue heterogeneity as for example within the tumor micro environment. The current invention describes a method (SUMI-Seq) which combines the use of Spatial Unique Molecular Identifier in situ sequencing and in vitro sequencing of rolonies derived from rolling circle amplification from padlock oligonucleotides targeting portion of RNA or cDNA transcript at a subcellular level with less limitation in the amount of transcripts and the length of the sequence that can be analyzed. Apart from padlocks oligonucleotides, the SUMI-Seq method can also be applied using circular oligonucleotides to spatially resolve proteins and metabolites to provide multiomics results.