The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Sep. 17, 2024
Filed:
Mar. 08, 2023
Paragon Genomics, Inc., Fremont, CA (US);
Zhitong Liu, Foster City, CA (US);
David Debruyne, Hayward, CA (US);
Jack Dong, San Ramon, CA (US);
Michael Clark, Pleasanton, CA (US);
Yutao Fu, San Marcos, CA (US);
Vidushi Kapoor, Fremont, CA (US);
Kalyani Patankar, Wheeling, IL (US);
Fang Xie, San Francisco, CA (US);
Logan Tom, Alameda, CA (US);
Paragon Genomics, Inc., Fremont, CA (US);
Abstract
Methods and compositions for making DNA libraries for massive parallel next generation sequencing (NGS), comprises two parts. These methods may be referred to as Triseq sequencing. The first part includes ligating a UMI adapter, amplifying the DNA fragments in the presence of dUTP, enriching the target molecules through primer extension by using a panel of both forward and reverse primers, and removing the dU-containing template DNA. The DNA molecules are organized to primary clones and subclones, labeled by the UMI on 5' and 3′ end of the DNA fragments, respectively. The second part includes sequencing the DNA library by NGS, deducing consensus sequence from each subclone, and from within each primary clone, and between the consensus sequences obtained from both forward and reverse primers.