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The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Patent No.:

US 12071646 B1

PDFFull Text
Date of Patent:
Aug. 27, 2024

Filed:

Dec. 28, 2021

Cells comprising mogroside pathway enzymes and uses thereof

Applicant:

The State of Israel, Ministry of Agriculture & Rural Development, Agricultural Research Organization (Aro)(volcani Center), Rishon-LeZion, IL;

Inventors:

Maxim Itkin, Kibbutz HaOgen, IL;

Rachel Davidovich-Rikanati, Alonei Abba, IL;

Shahar Cohen, Rishon-LeZion, IL;

Vitaly Portnoy, Nesher, IL;

Adi Doron-Faigenboim, Ramat-HaSharon, IL;

Marina Petreikov, Rishon-LeZion, IL;

Shmuel Shen, Moshav Beit-Elazari, IL;

Yaakov Tadmor, Timrat, IL;

Yosef Burger, Haifa, IL;

Efraim Lewinsohn, Timrat, IL;

Nurit Katzir, Kiryat-Tivon, IL;

Arthur A. Schaffer, Hashmonaim, IL;

Elad Oren, Beit Shearim, IL;

Assignee:

The State of Israel, Ministry of Agriculture & Rural Development, Agricultural Research Organization (ARO) (Volcani Center), Rishon-LeZion, IL;

Attorney:
Primary Examiner:

Delia M Ramirez

Int. Cl.
CPC ...
C12N 9/10 (2006.01); A23L 2/60 (2006.01); A23L 27/30 (2016.01); C07J 17/00 (2006.01); C12N 9/02 (2006.01); C12N 9/14 (2006.01); C12N 9/88 (2006.01); C12N 9/90 (2006.01); C12P 19/56 (2006.01); C12P 33/12 (2006.01); C12P 33/20 (2006.01);
U.S. Cl.
CPC ...
C12P 33/12 (2013.01); A23L 2/60 (2013.01); A23L 27/36 (2016.08); C07J 17/005 (2013.01); C12N 9/0071 (2013.01); C12N 9/0083 (2013.01); C12N 9/14 (2013.01); C12N 9/88 (2013.01); C12N 9/90 (2013.01); C12P 19/56 (2013.01); C12P 33/20 (2013.01); C12Y 114/99007 (2013.01); C12Y 204/02017 (2013.01); C12Y 402/01 (2013.01); C12Y 504/99033 (2013.01); A23V 2002/00 (2013.01);
Abstract

Isolated mogroside and mogrol biosynthetic pathway enzyme polypeptides useful in mogroside biosynthesis are provided. Mogroside biosynthetic pathway enzymes of the invention include squalene epoxidase (SE), epoxy hydratase (EH), cytochrome p450 (Cyp), cucurbitadienol synthase (CDS) and udp-glucosyl-transferase (UGT), Also provided are methods of producing a mogroside using the isolated mogroside and mogrol biosynthetic enzyme polypeptides, the methods comprising contacting a mogrol and/or a glycosylated mogrol (mogroside) with at least one UDP glucose glucosyl transferase (UGT) enzyme polypeptide of the invention catalyzing glucosylation of the mogrol and/or the glucosylated mogrol to produce a mogroside with an additional glucosyl moietie(s), thereby producing the mogroside. Alternatively or additionally provided is a method of synthesizing a mogrol, the method comprising contacting a mogrol precursor substrate with one or more mogrol biosynthetic pathway enzyme polypeptides as described herein catalyzing mogrol synthesis from the mogrol precursor substrate, thereby synthesizing the mogrol.

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