The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jun. 04, 2024
Filed:
Oct. 19, 2023
Jiangnan University, Wuxi, CN;
Jie Long, Wuxi, CN;
Ziying Ye, Wuxi, CN;
Zhengyu Jin, Wuxi, CN;
Cheng Lu, Wuxi, CN;
Xingfei Li, Wuxi, CN;
Yaoqi Tian, Wuxi, CN;
Yuxiang Bai, Wuxi, CN;
Xing Zhou, Wuxi, CN;
Chao Qiu, Wuxi, CN;
Long Chen, Wuxi, CN;
Zhengjun Xie, Wuxi, CN;
JIANGNAN UNIVERSITY, Wuxi, CN;
Abstract
Disclosed are an agarase mutant with improved thermal stability and application thereof, belonging to the fields of genetic engineering technology and enzyme engineering. The present disclosure provides an agarase mutant, which is obtained by mutating the amino acid at the 86site, the 373site, the 374site, the 496site, the 507site, or the 747site of agarase with an amino acid sequence as shown in SEQ ID NO. 1. The agarase mutant provided by the present disclosure improves the thermal stability and the hydrolytic activity of the agarase. Compared with the wild type enzyme, the mutant enzyme shows excellent heat resistance and can be industrially used at a relatively high temperature, so that the utilization rate of agar raw materials and the yield of oligosaccharides from agar are improved, and the mutant enzyme has a good industrial application prospect.