The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
May. 21, 2024

Filed:

Sep. 24, 2023
Applicants:

Vedabio, Inc., San Diego, CA (US);

The Board of Trustees of the University of Illinois, Urbana, IL (US);

Inventors:

Anurup Ganguli, San Diego, CA (US);

Ariana Mostafa, San Diego, CA (US);

Jacob Berger, San Diego, CA (US);

Ashish Pandey, San Diego, CA (US);

Assignees:
Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12Q 1/6823 (2018.01); C12N 9/22 (2006.01); C12N 15/11 (2006.01); C12Q 1/6806 (2018.01); C12Q 1/682 (2018.01);
U.S. Cl.
CPC ...
C12Q 1/6823 (2013.01); C12N 9/22 (2013.01); C12N 15/11 (2013.01); C12Q 1/6806 (2013.01); C12Q 1/682 (2013.01); C12N 2310/20 (2017.05); C12N 2310/315 (2013.01); C12N 2310/321 (2013.01); C12N 2310/3231 (2013.01); C12N 2320/10 (2013.01);
Abstract

The present disclosure describes compositions of matter comprising a ribonucleoprotein complex comprising a nucleic acid-guided nuclease and a guide RNA, and further comprising a blocking nucleic acid molecule represented by Formula IV, wherein Formula IV in the 5′-to-3′ direction comprises: T-D-M-A-Lp-C; wherein T is 17-31 nucleotides in length; D is 0-15 nucleotides in length; M is 1-25 nucleotides in length; A is 0-15 nucleotides in length and comprises a sequence complementary to D; and L is 3-25 nucleotides in length; p is 0 or 1; C is 4-15 nucleotides in length and comprises a sequence complementary to T; and wherein the blocking nucleic acid molecule comprises a sequence complementary to a gRNA.


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