The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jan. 16, 2024

Filed:

Jan. 22, 2018
Applicant:

Agency for Science, Technology and Research, Singapore, SG;

Inventors:

Jackie Y. Ying, Singapore, SG;

Leng Leng Chng, Singapore, SG;

Daniele Zink, Singapore, SG;

Aysha Farwin, Singapore, SG;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 5/00 (2006.01); C08F 293/00 (2006.01); C08L 53/00 (2006.01); C12N 5/0775 (2010.01);
U.S. Cl.
CPC ...
C12N 5/0075 (2013.01); C08F 293/005 (2013.01); C08L 53/005 (2013.01); C12N 5/0662 (2013.01); C08F 2438/00 (2013.01); C08F 2810/20 (2013.01); C08F 2810/50 (2013.01); C08L 2207/53 (2013.01); C08L 2312/00 (2013.01); C12N 2500/90 (2013.01); C12N 2500/98 (2013.01); C12N 2531/00 (2013.01); C12N 2533/30 (2013.01); C12N 2539/10 (2013.01);
Abstract

There is provided a polymeric microsphere comprising a thermally responsive monomer crosslinked with a functional group monomer, wherein the functional group monomer comprises at least one of a carboxylic acid functional group or an amine functional group. The thermally responsive monomer is preferably N-isopropylacrylamide (NIPAM), and the microspheres preferably comprise a coating of polymerized catecholamines (e.g. DOPA). There is also provided a method of preparing the polymeric microsphere and uses of the polymeric microsphere in culturing, harvesting, or expanding stem cells or stromal cells. Preferably, the cells, e.g. hMSCs (human mesenchymal stem/stromal cells), are expanded or harvested in serum-free and xeno-free medium.


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