The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Dec. 05, 2023

Filed:

Sep. 15, 2020
Applicant:

National Pingtung University of Science and Technology, Neipu, TW;

Inventors:

Hso-Chi Chaung, Neipu, TW;

Wen-Bin Chung, Neipu, TW;

Ann Ying-An Chen, Neipu, TW;

Mei-Li Wu, Neipu, TW;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 5/0783 (2010.01); G01N 33/50 (2006.01); C12N 5/0786 (2010.01); G01N 33/68 (2006.01); A01K 67/027 (2006.01);
U.S. Cl.
CPC ...
C12N 5/0638 (2013.01); A01K 67/0278 (2013.01); C12N 5/0637 (2013.01); C12N 5/0645 (2013.01); C12N 5/0646 (2013.01); G01N 33/5047 (2013.01); G01N 33/6869 (2013.01); A01K 2207/15 (2013.01); A01K 2217/206 (2013.01); A01K 2227/108 (2013.01); A01K 2267/0387 (2013.01);
Abstract

The present invention relates to an in vitro immune synapse system and a method of in vitro evaluating immune response using the same. The in vitro immune synapse system includes antigen-presenting cells (APCs) and at least one cell type of several specific T cell subtypes isolated from peripheral blood mononuclear cells (PBMCs), all of which is from a same individual of pigs. When a test sample is co-cultured in the in vitro immune synapse system for a given period, it can be determined that the test sample is immunogenic, immunostimulatory or not according to the immunization-related changes of these cells, thereby potentially replacing some kinds of animal experimentation.


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